Biologics
Biologics by Ligand Binding Assay (LBA)
QPS is at the forefront of a wide range of ligand binding assays for a variety of discovery research and pharmaceutical studies. The scientists at QPS have extensive experience in the development and validation of a variety of immunoassays under GLP guidelines. Our expertise enables us to offer our clients project-based and cost-effective immunoassay services, including:
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Assay development with colorimetric, fluorescence, chemiluminescence and electrochemiluminescence detections
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Assay validation under GLP regulations
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Sample analysis of target analyte in biological matrices to support preclinical and clinical programs
- QPS has been working on polypeptides and biologics using LC-MS/MS since 2000.
- The brute force approach is a combination of protein precipitation, SPE, LLE to extract, clean-up, and concentrate a standard LC-MS/MS methodology.
- The more elegant approach is immunoaffinity chromatography and LC-MS/MS. This methodology is essentially using an immunoaffinity column to trap the biologic, wash and back-flush onto a reverse-phase trapping column, wash and then back-flush onto an analytical column for LC-MS/MS.
- The decision on what methodology to use depends on the biologics. Both methodologies are non-trivial and highly experienced bioanalytical chemists are needed.
