Biologics

Biologics by Ligand Binding Assay (LBA)

QPS is at the forefront of a wide range of ligand binding assays for a variety of discovery research and pharmaceutical studies. The scientists at QPS have extensive experience in the development and validation of a variety of immunoassays under GLP guidelines. Our expertise enables us to offer our clients project-based and cost-effective immunoassay services, including:

 

  • Assay development with colorimetric, fluorescence, chemiluminescence and electrochemiluminescence detections

  • Assay validation under GLP regulations

  • Sample analysis of target analyte in biological matrices to support preclinical and clinical programs


  • Biologics by LC-MS/MS

  • QPS has been working on polypeptides and biologics using LC-MS/MS since 2000.
  • The brute force approach is a combination of protein precipitation, SPE, LLE to extract, clean-up, and concentrate a standard LC-MS/MS methodology.
  • The more elegant approach is immunoaffinity chromatography and LC-MS/MS. This methodology is essentially using an immunoaffinity column to trap the biologic, wash and back-flush onto a reverse-phase trapping column, wash and then back-flush onto an analytical column for LC-MS/MS.
  • The decision on what methodology to use depends on the biologics. Both methodologies are non-trivial and highly experienced bioanalytical chemists are needed.